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  4. Human glucagon-like peptide-1,GLP-1 ELISA Kit

Human glucagon-like peptide-1,GLP-1 ELISA Kit

  • 中文名称：
    人胰高血糖素样肽1(GLP-1)酶联免疫试剂盒
  • 货号：
    CSB-E08119h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO's GLP-1 human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GLP-1 in serum, plasma, or tissue homogenates. This assay uses an antibody specific to human GLP-1 coated on a 96-well plate. GLP-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated GLP-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of GLP-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

    GLP-1 is a gastrointestinal peptide that is produced by the enteroendocrine L cells and is released in response to meal intake. GLP-1 mainly functions to stimulate insulin secretion and to suppress food intake, gastric emptying, and glucagon secretion, thereby limiting postprandial glucose excursions. It is important for glucose homeostasis. It also inhibits gastrointestinal motility and secretion and thus acts as an enterogastrone and part of the "ileal brake" mechanism. GLP-1 stimulates the proliferation, differentiation, and neogenesis of β cells.

  • 别名：
    N/A
  • 缩写：
    GLP-1
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    2.5 ng/mL-40 ng/mL
  • 灵敏度：
    0.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 87-107  
    1:2 Average % 96  
    Range % 90-106  
    1:4 Average % 95  
    Range % 86-105  
    1:8 Average % 96  
    Range % 89-100  
  • 回收率：
    The recovery of human GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-101  
    EDTA plasma (n=4) 95 89-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2.3 0.063 0.061 0.062    
    4.6 0.169 0.172 0.171    
    9.1 0.398 0.384 0.391    
    22.9 0.861 0.872 0.867    
    40 1.660 1.653 1.657    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

关于CSB-E08119h人源胰高血糖素样肽-1（GLP-1）ELISA试剂盒，请问我们应该如何采集血样，是否需要在EDTA管中添加DPP-4抑制剂？ 在Cusabio的说明中，并没有说明在采集血浆样本时需要添加DPP-4抑制剂。如果我们使用Cusabio的ELISA试剂盒，是否真的不需要向样本中添加DPP-4抑制剂呢？

A:
大多数CUSABIO ELISA试剂盒都是用于检测天然样本的。当我们检测血清或血浆样本时，通常不会进行特殊处理，例如向样本中添加DPP-4抑制剂。尽管一些参考文献指出DPP-4抑制剂（二肽基肽酶4抑制剂）是治疗2型糖尿病的一种药物，可以抑制胰高血糖素样肽-1（GLP-1）和葡萄糖依赖性胰岛素促泌肽（GIP）的失活，并增加内源性GLP-1和GIP的水平。但我们从未尝试过这种操作，不确定结果会是什么。