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  4. Recombinant Mouse Cytochrome P450 1A2 (Cyp1a2)

Recombinant Mouse Cytochrome P450 1A2 (Cyp1a2)

  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A2(Cyp1a2),Yeast
  • 货号：
    CSB-YP006396MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A2(Cyp1a2),Yeast
  • 货号：
    CSB-EP006396MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A2(Cyp1a2),Yeast
  • 货号：
    CSB-EP006396MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A2(Cyp1a2),Yeast
  • 货号：
    CSB-BP006396MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A2(Cyp1a2),Yeast
  • 货号：
    CSB-MP006396MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp1a2
  • Uniprot No.：
  • 别名：
    Cyp1a2; Cyp1a-2Cytochrome P450 1A2; EC 1.14.14.1; CYPIA2; Cholesterol 25-hydroxylase; Cytochrome P450-P2; Cytochrome P450-P3; Hydroperoxy icosatetraenoate dehydratase; EC 4.2.1.152
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-513
  • 氨基酸序列
    AFSQYISLA PELLLATAIF CLVFWMVRAS RTQVPKGLKN PPGPWGLPFI GHMLTVGKNP HLSLTRLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVRQ GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS DPTSASSCYL EEHVSKEANH LVSKLQKAMA EVGHFEPVSQ VVESVANVIG AMCFGKNFPR KSEEMLNIVN NSKDFVENVT SGNAVDFFPV LRYLPNPALK RFKTFNDNFV LFLQKTVQEH YQDFNKNSIQ DITSALFKHS ENYKDNGGLI PEEKIVNIVN DIFGAGFDTV TTAITWSILL LVTWPNVQRK IHEELDTVVG RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP KERCIYINQW QVNHDEKQWK DPFVFRPERF LTNNNSAIDK TQSEKVMLFG LGKRRCIGEI PAKWEVFLFL AILLQHLEFS VPPGVKVDLT PNYGLTMKPG TCEHVQAWPR FSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.
  • 基因功能参考文献：
    1. Distribution of parent PCB congeners also varied by genotype with strikingly high levels of PCB 77 in poor-affinity Ahr (d) Cyp1a2(-/-) while Ahr (b) Cyp1a2(+/+) mice effectively sequestered coplanar PCBs in the liver. PMID: 29197979
    2. our results suggest a more important contribution of CYP1A2 to the in vivo plasma clearance and thus detoxification of 4-Aminobiphenyl PMID: 25922528
    3. hepatic CYP1A2 plays a critical role in the attenuation of hyperoxic lung injury by decreasing lipid peroxidation and oxidative stress in vivo. PMID: 25680282
    4. Results indicate Cyp1a2 genotype is important in susceptibility to PCB-induced deficits in learning and memory. PMID: 22935098
    5. Miroestrol and deoxymiroestrol significantly up-regulated CYP2B9 while suppressing CYP1A2 at both transcriptional and enzymatic levels. PMID: 22260863
    6. A report of uroporphyria development in Cyp1a2-/- mice additionally null for both alleles of the hemochromatosis (Hfe) gene and heterozygous for deletion of the uroporphyrinogen decarboxylase (Urod) gene (genotype: Cyp1a2-/-;Hfe-/-;Urod+/-). PMID: 21880518
    7. High levels of maternal hepatic CYP1A2 protect against deficits in learning and memory in offspring exposed to a mixture of coplanar and noncoplanar PCBs; high-affinity AHR is the next most important factor in protection of offspring. PMID: 21571617
    8. In rodents, aristolochic acid demethylation, mediated by CYP1A2, is a primary pathway of aristolochic acid detoxication. PMID: 20164109
    9. The expression of Cyp1a1, Cyp1a2, and OGG1genes was significantly up-regulated in mice given diets containing 375 ppm dicyclanil or more. PMID: 20118626
    10. the TRE element in E1 is essential for constitutive expression of the mouse Cypla2 gene PMID: 12372429
    11. Liver-specific CYP1A2 plays an important role in persistent induction of CYP1A1 in liver, but not lung. PMID: 12438513
    12. CYP1A2 is not required for TCDD-induced immunosuppression in the mouse. PMID: 15003330
    13. Cyp1a2 is regulated by histone deacetylase inhibitors in mouse hepatocytes. PMID: 15252868
    14. A link not only between CYP1A2, iron metabolism, and the induction of uroporphyria by polychlorinated biphenyls, but also with subsequent hepatocarcinogenesis. PMID: 15845371
    15. maternal hepatic CYP1A2, by sequestering dioxin and thus altering the pharmacokinetics, protects the embryos from toxicity and birth defects PMID: 16636061
    16. cytochromes P4501A2 and 2E1 catalytic activities are inhibited by natural analogues of trans-resveratrol PMID: 16684708
    17. Highly conserved in mice and not to be associated with interstrain differences in constitutive CYP1A2 enzyme activity. PMID: 17569574
    18. Data show that tea polyphenols reduced CYP2E1 and CYP1A2 expression at both protein and mRNA levels in a dose-dependent manner, and suggest that they possess potential hepatoprotective properties. PMID: 19370779
    19. The results demonstrate that caffeine alters the striatal CYP1A2, adenosine A(2A) receptor and DAT expressions in mice exposed to MPTP. PMID: 19520064

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: mmu:13077

    STRING: 10090.ENSMUSP00000034860

    UniGene: Mm.15537