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  4. Recombinant Mouse E3 ubiquitin-protein ligase XIAP (Xiap)

Recombinant Mouse E3 ubiquitin-protein ligase XIAP (Xiap)

  • 货号：
    CSB-YP726691MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP726691MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP726691MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP726691MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP726691MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Xiap; Aipa; Api3; Birc4; Miha; E3 ubiquitin-protein ligase XIAP; EC 2.3.2.27; Baculoviral IAP repeat-containing protein 4; IAP homolog A; Inhibitor of apoptosis protein 3; IAP-3; mIAP-3; mIAP3; RING-type E3 ubiquitin transferase XIAP; X-linked inhibitor of apoptosis protein; X-linked IAP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-496
  • 氨基酸序列
    MTFNSFEGTR TFVLADTNKD EEFVEEFNRL KTFANFPSSS PVSASTLARA GFLYTGEGDT VQCFSCHAAI DRWQYGDSAV GRHRRISPNC RFINGFYFEN GAAQSTNPGI QNGQYKSENC VGNRNPFAPD RPPETHADYL LRTGQVVDIS DTIYPRNPAM CSEEARLKSF QNWPDYAHLT PRELASAGLY YTGADDQVQC FCCGGKLKNW EPCDRAWSEH RRHFPNCFFV LGRNVNVRSE SGVSSDRNFP NSTNSPRNPA MAEYEARIVT FGTWTSSVNK EQLARAGFYA LGEGDKVKCF HCGGGLTDWK PSEDPWEQHA KWYPGCKYLL DEKGQEYINN IHLTHSLEES LGRTAEKTPS LTKKIDDTIF QNPMVQEAIR MGFSFKDIKK TMEEKIQTSG SSYLSLEVLI ADLVSAQKDN TEDESSQTSL QKDISTEEQL RRLQEEKLCK ICMDRNIAIV FVPCGHLVTC KQCAEAVDKC PMCYTVITFK QKIFMS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.
  • 基因功能参考文献：
    1. mRNA and protein expressions of XIAP were decreased via siRNA targetting, which leads to increases in cell apoptosis and caspase-3 and caspase-9 activity. It also contributed to the reduced tumor size and tumor weight in a nude mice model of esophageal cancer. PMID: 28821565
    2. The neuron-specific form of FAIM protein (FAIM-L) is a death receptor antagonist that stabilizes XIAP protein levels, thus preventing death receptor-induced neuronal apoptosis. Here we show that FAIM-L modulates synaptic transmission, prevents chemical-LTD induction in hippocampal neurons, and thwarts axon degeneration after nerve growth factor (NGF) withdrawal. PMID: 27767058
    3. These data reveal how, upon XIAP deficiency, a TLR-TNF-TNFR2 axis drives cIAP1-TRAF2 degradation to allow TLR or TNFR1 activation of RIPK3-caspase-8 and IL-1beta. This mechanism may explain why XIAP-deficient patients can exhibit symptoms reminiscent of patients with activating inflammasome mutations. PMID: 28723569
    4. There was a significantly decreased percentage of IL-17A-producing CD4 T cells in mice receiving Tregs from xIAP mice. xIAP appears dispensable for the generation of induced Treg cells as well as function of natural Treg cells. There appeared to be a role of xIAP in generation of IL-17-producing cells from either naive CD4 T cells or Treg cells. PMID: 26825770
    5. Drugs targeting XIAP and cIAP1/2 may be effective for osteosarcoma patients whose tumors express abundant RIPK1 and contain high levels of TNFalpha. PMID: 27129149
    6. Deletion of XIAP switches cell death away from necrosis to apoptosis. PMID: 28300832
    7. These results indicate that XIAP plays an important physiologic role in regulating sublethal CASP-3 activity within central neurons and thereby facilitates synaptic plasticity and memory acquisition. PMID: 27189977
    8. XIAP antagonizes the switch from TNFalpha-induced apoptosis to necroptosis in mouse neutrophils. PMID: 27735938
    9. Results show that XIAP binds to the C terminus of Ptch1 and mediates the death-dependent function of Ptch1. PMID: 25292199
    10. XIAP controls RIP3-dependent cell death and IL-1beta secretion in response to TNF. PMID: 24882010
    11. XIAP deficiency selectively impaired B-cell chronic lymphocytic leukemia/lymphoma 10 (BCL10)-mediated innate responses to dectin-1 ligands but did not affect responses to various Toll-like receptor agonists. PMID: 25190756
    12. XIAP mRNA level was also reduced in the BRE-depleted cells, but the level of reduction was less profound than that of the protein level. However, BRE could not delay protein turnover of XIAP. PMID: 24395041
    13. Identify xIAP and cIAP1 as molecular targets of ceramide and show ceramide analog LCL85 is an effective sensitizer in overcoming resistance of metastatic colon and breast cancers to apoptosis induction to suppress metastasis in vivo. PMID: 24422988
    14. XIAP modulates ubiquitylation of RIP1 and suppresses RIP3-dependent cell death and inflammasome activation in response to TNF-signaling in innate immune cells. PMID: 24882010
    15. XIAP-/- DRG sensory neurons degenerate more rapidly and contain more active caspase-3. PMID: 23954782
    16. XIAP ubiquitylates RIPK2 and recruits the linear ubiquitin chain assembly complex to NOD2 PMID: 22607974
    17. XIAP is expressed in oligodendrocytes in vivo and in vitro. Increased XIAP expression is associated with protection against selected cell death pathways, whereas decreased expression increases oligodendroglial cell death. PMID: 22065417
    18. ARTS (Septin 4) promotes apoptosis mainly through binding and antagonizing XIAP. PMID: 21695558
    19. A critical role is identified for XIAP in regulating neuronal apoptosis in neonatal brain injury. PMID: 19570023
    20. XIAP-TAB1-TAK1 complex is dependent on NRAGE for IKK-alpha/beta phosphorylation and NF-kappaB activation. PMID: 21789165
    21. Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation. PMID: 21712378
    22. XIAP is a previously uncharacterized target for miR-23a. miR-23a levels differed in male and female ischemic brains, providing evidence for sex-specific miRNA expression in stroke. PMID: 21709246
    23. Ex vivo XIAP gene transfer in islets prior to transplantation has the potential to increase the number of donor islets available for transplantation and may enhance graft function and long-term transplant success. PMID: 21099289
    24. Transgenic mice overexpress XIAP and develop hearing loss considerably more slowly than their wild-type C57BL/6J littermates, primarily within the high-frequency range. PMID: 18755525
    25. Data demonstrated a role of XIAP for the integrity of both innate and cellular immune responses during C. pneumoniae infection. PMID: 20427267
    26. Xiap-ps1 is a pseudogene generated by retro-transposition of a spliced Xiap message to chromosome 7 PMID: 19956646
    27. the inhibition of apoptosis by BMP2 and GDF5 does not depend on more complex signal transduction pathways such as smad and MAPK signaling but on direct stabilization of XIAP by BMPR2. PMID: 19782107
    28. Membrane-associated XIAP induces mitochondrial outer membrane permeabilization leading to cytochrome c and Smac release, which is dependent on Bax and Bak. PMID: 19875445
    29. p53 activation enhances XIAP inhibition-induced cell death by promoting mitochondrial release of second mitochondria-derived activator of caspases (SMAC) and by inducing the expression of caspase-6. PMID: 19897582
    30. Transient focal ischemia was produced for 1 hour in mice. The subcellular localization of XIAP became more extensive within the cells during reperfusion, as compared with the normal state. PMID: 12395105
    31. dopamine neurons of NSE-xiap mice were resistant to the damaging effects of the dopaminergic neurotoxin MPTP. MPTP-induced reduction of striatal dopamine metabolism was also attenuated in NSE-xiap mice PMID: 12667469
    32. XIAP transgenic animals exhibited less brain damage, less reduction in brain protein synthesis, and less active caspase-3 after ischemia. PMID: 12812761
    33. These results suggest that interaction among XIAP, Smac/DIABLO, and caspase-9 plays an important role in the regulation of apoptotic neuronal cell death after transient focal cerebral ischemia. PMID: 12973017
    34. The removal of XIAP inhibition causes cytochrome c to activate caspases in sympathetic neurons, suggesting a critical function of XIAP in regulating apoptosis. PMID: 14623868
    35. XIAP interacts with CHEK1 during mitosis. PMID: 14759516
    36. Combination of radiotherapy and inhibition of survivin and XIAP through the antisense approach results in improved tumor control by radiotherapy in a mouse model of lung cancer. PMID: 15258565
    37. During pregnancy, alveolar differentiation in the mammary gland is defective in XIAP mutants, but the mice are still capable of lactation. PMID: 15540113
    38. XIAP overexpression in islet beta-cells enhances engraftment and minimizes hypoxia-reperfusion injury PMID: 15888033
    39. It is reported report that ras oncogene, an established inhibitor of anoikis, triggers a significant upregulation of anti-apoptotic proteins cIAP2 and XIAP in intestinal epithelial cells. PMID: 16115895
    40. XIAP inhibits JNK1 activation by TGF-beta1 through ubiquitin-mediated proteosomal degradation of TAK1 PMID: 16157589
    41. Data show that overexpression of X-linked Inhibitor of Apoptosis Protein (XIAP)inhibits caspase-12 cleavage and reduces calpain activity in amyotrophic lateral sclerosis mice. PMID: 16566922
    42. Microarray, real-time PCR, and immunohistochemical analyses of skin of downless mice revealed a strong decrease of expression of X-linked inhibitor of apoptosis protein (XIAP), compared with the controls, suggesting XIAP as a target for Edar signaling. PMID: 17148670
    43. Notch inhibits apoptosis by direct interference with XIAP ubiquitination and degradation. PMID: 17318174
    44. Caspase 9 activation in response to intrinsic apoptotic stimuli can be uncoupled from Apaf-1 in vivo by XIAP antagonists. PMID: 17562856
    45. Results from the TRAMP mouse prostate cancer model suggest that alternative mechanisms of apoptosis resistance play a significant oncogenic role in the setting of Xiap deficiency. PMID: 18259199
    46. Data suggest that increased levels of hnRNP C1/C2 may modulate XIAP translation by interacting with the XIAP-IRES, and may foster synthesis of XIAP as a protective pathway by which neurons try to counteract the initial deleterious effects of apoptosis. PMID: 18363099
    47. murine cells lacking XIAP and c-IAP 2, but not c-IAP1, exhibit heightened apoptotic sensitivity to both intrinsic and extrinsic apoptotic stimuli. PMID: 18684108
    48. XIAP is an important apoptotic regulator in experimental autoimmune encephalomyelitis PMID: 18687476
    49. These findings reveal that XIAP prevents prolonged JNK activation and is critically involved in modulating ROS levels through regulation of antioxidative genes, thereby inhibiting ROS-induced apoptosis. PMID: 18692482
    50. Through interaction with MEKK2, XIAP functions in an ubiquitin ligase dependent manner to evoke a second wave of NF-kappaB activation, resulting in the modulation of NF-kappaB target gene expression. PMID: 18761086

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    IAP family
  • 数据库链接：

    KEGG: mmu:11798

    STRING: 10090.ENSMUSP00000026978

    UniGene: Mm.259879