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  4. Recombinant Mouse Nanos homolog 2 (Nanos2)

Recombinant Mouse Nanos homolog 2 (Nanos2)

  • 中文名称：
    小鼠Nanos2重组蛋白
  • 货号：
    CSB-YP015437MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nanos2重组蛋白
  • 货号：
    CSB-EP015437MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nanos2重组蛋白
  • 货号：
    CSB-EP015437MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nanos2重组蛋白
  • 货号：
    CSB-BP015437MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nanos2重组蛋白
  • 货号：
    CSB-MP015437MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nanos2
  • Uniprot No.：
  • 别名：
    Nanos2; Nos2; Nanos homolog 2; NOS-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-136
  • 氨基酸序列
    MDLPPFDMWR DYFNLSQVVM DIIQSRKQRQ EGEVAEEPNS RPQEKSEQDL EGYPGCLPTI CNFCKHNGES RHVYTSHQLK TPEGVVVCPI LRHYVCPLCG ATGDQAHTLK YCPLNSSQQS LYRRSGRNSA GRRVKR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.
  • 基因功能参考文献：
    1. Data show that miR-34c up-regulated Nanos2 in mouse model of cryptorchidism. PMID: 30322389
    2. On the other hand, loss of NANOS2 results in the up-regulation of GFRA1 and PLZF, indicating a precocious entry of GST. Taken together, our data suggest that NANOS2 functions as an intrinsic timekeeper of GST in the mouse testes PMID: 29339164
    3. show that Nanos2, an evolutionarily conserved RNA-binding protein, works with other cellular messenger ribonucleoprotein (mRNP) components to ensure the primitive status of spermatogonial stem cells PMID: 26120033
    4. Our experiments identified that miR-34c may promote meiosis process by interacting with Nanos2 leading up-regulation of Stra8 in mouse spermatogonial stem cells. PMID: 24038201
    5. NANOS2 is not merely a suppressor of meiosis, but instead plays pivotal roles in the sexual differentiation of male germ cells. PMID: 24183939
    6. NANOS2 mutant lacking these first 10 amino acids fails to bind CCR4-NOT deadenylation complex which is essential for male germ cell development. PMID: 22448252
    7. we suggest that NANOS2 acts downstream of GDNF signaling to maintain undifferentiated state of spermatogonial stem cells. PMID: 22102605
    8. Nanos2 promotes male germ cell differentiation, while simultaneously suppressing a female program. PMID: 20652721
    9. Nanos2 is continuously expressed in male germ cells from fetal gonocytes to postnatal spermatogonial stem cells. PMID: 20159962
    10. NANOS2/CCR4-NOT complex has deadenylase activity in vitro, and some of the RNAs implicated in meiosis interact with NANOS2 and are accumulated in its absence. PMID: 20133598
    11. Both in baseline conditions and during endotoxemia, the absence of NOS2 upregulated NOS1 and/or NOS3, which increased intestinal metabolism. PMID: 14656712
    12. nanos2 plays a role in the maintenance and differentiation of gonocytes/spermatogonia in neonatal testes PMID: 16806845
    13. study found NANOS2 is essential for maintaining spermatogonial stem cells; NANOS2 is a key stem cell regulator that is expressed in self-renewing spermatogonial stem cells and maintains the stem cell state during murine spermatogenesis PMID: 19745153

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, P-body. Cytoplasm, perinuclear region.
  • 蛋白家族：
    Nanos family
  • 组织特异性：
    Predominantly expressed in male germ cells. Expressed in self-renewing spermatogonial stem cells and developing gonads.
  • 数据库链接：

    KEGG: mmu:378430

    STRING: 10090.ENSMUSP00000069765

    UniGene: Mm.277153