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  4. Recombinant Mouse Neurogenin-3 (Neurog3)

Recombinant Mouse Neurogenin-3 (Neurog3)

  • 中文名称：
    小鼠Neurog3重组蛋白
  • 货号：
    CSB-YP015732MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Neurog3重组蛋白
  • 货号：
    CSB-EP015732MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Neurog3重组蛋白
  • 货号：
    CSB-EP015732MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Neurog3重组蛋白
  • 货号：
    CSB-BP015732MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Neurog3重组蛋白
  • 货号：
    CSB-MP015732MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Neurog3; Ath4b; Atoh5; Ngn3; Neurogenin-3; NGN-3; Helix-loop-helix protein mATH-4B; mATH4B; Protein atonal homolog 5
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-214
  • 氨基酸序列
    MAPHPLDALT IQVSPETQQP FPGASDHEVL SSNSTPPSPT LIPRDCSEAE VGDCRGTSRK LRARRGGRNR PKSELALSKQ RRSRRKKAND RERNRMHNLN SALDALRGVL PTFPDDAKLT KIETLRFAHN YIWALTQTLR IADHSFYGPE PPVPCGELGS PGGGSNGDWG SIYSPVSQAG NLSPTASLEE FPGLQVPSSP SYLLPGALVF SDFL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types.
  • 基因功能参考文献：
    1. Dorsal pancreas dysgenesis and ectopic pancreas is Neurog3 dependent PMID: 30093553
    2. Our data show that chemical inhibition of the H3K27me3 demethylases Jmjd3/Utx blunts Neurogenin3-dependent gene activation in vitro. Conversely, inhibition of the H3K27me3 methyltransferase Ezh2 enhances both the transactivation ability of Neurogenin3 in cultured cells and the formation of insulin-producing cells during directed differentiation from pluripotent cells PMID: 29530603
    3. This reviews the expression and function of NEUROG3 in both mouse and human pancreatic development. [Review Article] PMID: 27615127
    4. Data suggest that hepatocytes can be reprogrammed into insulin-producing cells in vivo by transfection of neurogenin-3, Pdx1, and MafA genes using non-viral hydrodynamics injection; this procedure was used in treatment of streptozotocin diabetes; fasting blood glucose was reduced to normal. (Pdx1 = pancreatic and duodenal homeobox 1; MafA = v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A) PMID: 28100871
    5. Neurogenin3 controls its ability to promote pancreatic endocrine differentiation and to maintain beta cell function in the presence of pro-proliferation cues PMID: 28457793
    6. Phosphorylation of NEUROG3 links endocrine differentiation to the cell cycle in pancreatic progenitors PMID: 28441528
    7. limiting Neurog3 expression dramatically increased the proportional representation of Sox9(+) Neurog3(TA.LO) progenitors, with a doubling of its mitotic index relative to normal Neurog3 expression, suggesting that low Neurog3 expression is a defining feature of this cycling endocrine-biased state PMID: 27585590
    8. inflammatory cytokine insults stimulate epithelial-to-mesenchymal transition (EMT) as well as the endocrine program in human pancreatic ductal cells via STAT3-dependent NGN3 activation. PMID: 27068459
    9. Duplication of pre-existing beta-cells is not the sole source of new beta-cells during pregnancy; Ngn3 may be involved in this process. PMID: 24940737
    10. data demonstrate that HIF1-alpha negatively controls beta cell differentiation in vivo by regulating NGN3 expression, and that this effect is mediated by signals from blood vessels. PMID: 25186293
    11. Data indicate that all neurogenin 3 (Neurog3)-tuberous sclerosis complex 1 (Tsc1)-/- mice developed notable adenocarcinoma-like lesions in pancreas starting from the age of 100 days old. PMID: 25425965
    12. Activation of the developmental pathway neurogenin-3/microRNA-7a regulates cholangiocyte proliferation in response to injury. PMID: 24925797
    13. Hnf1b plays a crucial role in the regulatory networks that control pancreatic MPC expansion, acinar cell identity, duct morphogenesis and generation of endocrine precursors. PMID: 25715395
    14. The role of neurogenin 3 and PD-L1 in the genesis of neo-islets in NOD mice is reported. PMID: 25332429
    15. the prophylactic administration of Ad-hNgn3 before diabetic induction protected mice from developing hyperglycemia PMID: 24635696
    16. Neurog3 establishes the posterior boundary of the serotonergic system by actively suppressing serotonergic specification in the spinal cord. PMID: 25392491
    17. Insm1 works to promote endocrine cell differentiation in a pathway that involves Neurog3 and Ripply3 PMID: 25053427
    18. three transcription factors, PDX1, NGN3 and MAFA, are very important in pancreatic development. Overexpression of these three factors can reprogram both pancreatic exocrine and SOX9-positive cells of the liver into cells resembling pancreatic beta cells PMID: 24312421
    19. The role of Ngn3 in the specification of hypothalamic neurons controlling energy balance, is reported. PMID: 23649822
    20. K cells could be reprogrammed partially to beta-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture. PMID: 24365150
    21. Ngn3 activation in duct cells is not a signature for adult beta cell neogenesis. PMID: 23867457
    22. Cytoplasmic Ngn3 is associated with microtubules and important for neuronal development. PMID: 23383123
    23. these data uncover the potential functional cooperation between the endocrine lineage-determining factor Neurogenin3 and the widespread endoderm progenitor factor Foxa2 in the implementation of the endocrine developmental program in the pancreas. PMID: 23471965
    24. Notch-mediated post-translational control of Ngn3 protein stability regulates pancreatic patterning and cell fate commitment. PMID: 23370147
    25. The results established a mechanism by which activation of STAT3 regulates the expression of NEUROG3 to subsequently drive differentiation of spermatogonial stem cells and progenitor spermatogonia. PMID: 22378757
    26. Although Sox9 is required for Ngn3 induction, endocrine differentiation necessitates subsequent Sox9 downregulation and evasion from Notch activity via cell-autonomous repression of Sox9 by Ngn3. PMID: 22675211
    27. mechanism of transdifferentiation of pancreatic exocrine cells to beta (beta) cells in vivo by Ngn3 PMID: 22150363
    28. The results indicate that GLIS3 controls fetal islet differentiation via direct transactivation of Neurog3, a perturbation that causes neonatal diabetes in mice. PMID: 21786021
    29. Forced expression of PLAG1 under the control of the Pdx1 or Ngn3 promoter in murine pancreas induces different degrees of endocrine hormone imbalances. PMID: 21294959
    30. Findings show that the utility of manipulating Pdx1 and Ngn3 expression in a stage-specific manner as an important new strategy for the efficient generation of functionally immature insulin-producing beta-islet cells from ES cells. PMID: 21931641
    31. These results reveal a previously unknown involvement of Formin1 in dendritogenesis and synaptogenesis and indicate that this protein is a key component of the Ngn3 signaling pathway that controls neuronal differentiation. PMID: 21818269
    32. Neurogenin3 can trigger the conversion of Math6 from a poorly transcribed bivalent to an active monovalent state in vitro, hence providing a mechanism whereby Neurogenin3 may activate Math6 in endocrine progenitors PMID: 21676531
    33. Ngn3 deficiency results in reduced branching and enlarged pancreatic duct-like structures, concomitant with Ngn3 promoter activation throughout the ductal epithelium and reduced Notch signaling PMID: 21888903
    34. Data demonstrate that Ngn3 indirectly controls Snail2 protein expression post-transcriptionally to repress E-cadherin. PMID: 21287656
    35. These results suggest that oestradiol regulates neurogenin 3 in primary hippocampal neurones by a nonclassical steroid signalling mechanism, which involves the down-regulation of Notch activity. PMID: 21251092
    36. Results suggest that Insm2 plays an important role in the differentiation cascade of Ngn3/NeuroD1 signaling in pancreatic islets. PMID: 21343251
    37. Neurog3 is essential for tuft cell differentiation. PMID: 21383077
    38. Cdk4 promotes pancreatic beta cell development by directing E2f1-mediated activation of Ngn3 and increasing the pool of endocrine precursors PMID: 21490060
    39. Neurogenin3 inhibits proliferation in pancreatic endocrine progenitors by inducing Cdkn1a. PMID: 21173230
    40. Ngn3+ progenitors contribute to subsets of POMC, NPY, TH and SF1 neurons and is required for the specification of these neuronal subtypes in the ventral hypothalamus. PMID: 21074524
    41. GDNF stimulates increased expression of transcription factors including Ngn3 during the embryonic stages of pancreas development. PMID: 20448145
    42. blocking ngn-3 expression in the E11.5 embryonic pancreas results in reduced endocrine differentiation. PMID: 20471370
    43. study report localization & subcellular distribution of Ngn3 in the hippocampus in vivo & in neuronal cultures; results suggest that Ngn3 may play a role in neuronal development by actions in the cytoplasm PMID: 20235092
    44. Thus, up-regulation of the hypothalamic NPY system is probably a feature characteristic of Ngn3 null mice. PMID: 20127365
    45. OVOL1, a downstream target of NGN3, may play an important role in regulating the balance between proliferation and differentiation of pancreatic cells PMID: 19882138
    46. control of Neurog3+ cell number and the Neurog3 threshold-dependent endocrine differentiation mechanism combine to select a specific proportion of pancreatic progenitor cells to adopt the islet cell fate PMID: 20025861
    47. Rfx6 is dependent on Ngn3 and acts upstream of or in parallel with NeuroD, Pax4 and Arx transcription factors during islet cell differentiation. PMID: 20040487
    48. NGN3+ cells are islet progenitors during embryogenesis and in adult mice. PMID: 11973276
    49. Neurogenin 3 is essential for the proper specification of gastric enteroendocrine cells and the maintenance of gastric epithelial cell identity PMID: 12080087
    50. Neurogenin3 and HNF1alpha activate the pax4 gene during the cascade of gene expression events that control pancreatic endocrine cell development PMID: 12837760

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:11925

    STRING: 10090.ENSMUSP00000054054

    UniGene: Mm.57236