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  4. Recombinant Mouse Sperm protamine P1 (Prm1)

Recombinant Mouse Sperm protamine P1 (Prm1)

  • 中文名称：
    小鼠Prm1重组蛋白
  • 货号：
    CSB-YP018728MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Prm1重组蛋白
  • 货号：
    CSB-EP018728MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Prm1重组蛋白
  • 货号：
    CSB-EP018728MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Prm1重组蛋白
  • 货号：
    CSB-BP018728MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Prm1重组蛋白
  • 货号：
    CSB-MP018728MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Prm1
  • Uniprot No.：
  • 别名：
    Prm1; Prm-1; Sperm protamine P1; Cysteine-rich protamine
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-51
  • 氨基酸序列
    ARYRCCRSK SRSRCRRRRR RCRRRRRRCC RRRRRRCCRR RRSYTIRCKK Y
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.
  • 基因功能参考文献：
    1. Data show that male mice from lines showing moderate transgene expression were fully fertile whereas strong overexpression of the Prm1-EGFP fusion protein resulted in complete and dominant male sterility. PMID: 20095053
    2. docking of protamine 1 to the nuclear envelope is an important intermediate step in spermiogenesis PMID: 14701833
    3. Germ cell nuclear factor acts as a repressor of basal transcription on both the Protamine 1 and Protamine 2 promoters. PMID: 15236322
    4. JHDM2A directly binds to and controls the expression of transition nuclear protein 1 (Tnp1) and protamine 1 (Prm1) genes, the products of which are required for packaging and condensation of sperm chromatin PMID: 17943087
    5. Study shows that protamine 1 and 2 mRNAs, which are specific to the round spermatid stage, are repressed at the two-pronuclei and two-cell stages postfertilization, respectively, in embryos created by round spermatid injection, by distinct mechanisms. PMID: 18343090

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Protamine P1 family
  • 组织特异性：
    Testis.
  • 数据库链接：

    KEGG: mmu:19118

    STRING: 10090.ENSMUSP00000023144

    UniGene: Mm.42733