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  4. Recombinant Rat Histone-lysine N-methyltransferase PRDM9 (Prdm9)

Recombinant Rat Histone-lysine N-methyltransferase PRDM9 (Prdm9)

  • 中文名称：
    大鼠Prdm9重组蛋白
  • 货号：
    CSB-YP018652RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Prdm9重组蛋白
  • 货号：
    CSB-EP018652RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Prdm9重组蛋白
  • 货号：
    CSB-EP018652RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Prdm9重组蛋白
  • 货号：
    CSB-BP018652RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Prdm9重组蛋白
  • 货号：
    CSB-MP018652RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Prdm9
  • Uniprot No.：
  • 别名：
    Prdm9; Histone-lysine N-methyltransferase PRDM9; PR domain zinc finger protein 9; PR domain-containing protein 9; Protein-lysine N-methyltransferase PRDM9; EC 2.1.1.-; [histone H3]-lysine36 N-trimethyltransferase PRDM9; EC 2.1.1.359; [histone H3]-lysine4 N-trimethyltransferase PRDM9; EC 2.1.1.354; [histone H3]-lysine9 N-trimethyltransferase PRDM9; EC 2.1.1.355; [histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9; EC 2.1.1.362; [histone H4]-lysine20 N-methyltransferase PRDM9; EC 2.1.1.361
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-796
  • 氨基酸序列
    MSRTMNTNKP EENSTEGDAG KLEWKPKVKD EFKDISIYFS KEEWAEMGEW EKIRYRNVKR NYKMLISIGL RAPRPAFMCY QRQAIKPQIN DNEDSDEEWT PKQQVSSPWV PFRVKHSKQQ KETPRMPLSD KSSVKEVFGI ENLLNTSGSE HAQKPVCSPE EGNTSGQHFG KKLKLRRKNV EVNRYRLRER KDLAYEEVSE PQDDDYLYCE KCQNFFIDSC PNHGPPVFVK DSVVDRGHPN HSVLSLPPGL RIGPSGIPEA GLGVWNEASD LPVGLHFGPY KGQITEDEEA ANSGYSWLIT KGRNCYEYVD GQDESQANWM RYVNCARDDE EQNLVAFQYH RKIFYRTCRV IRPGRELLVW YGDEYGQELG IKWGSKMKKG FTAGRELRTE IHPCFLCSLA FSSQKFLTQH VEWNHRTEIF PGASARINPK PGDPCPDQLQ EHFDSQNKND KASNEVKRKS KPRHKWTRQR ISTAFSSTLK EQMRSEESKR TVEEELRTGQ TTNIEDTAKS FIASETSRIE RQCGQCFSDK SNVSEHQRTH TGEKPYICRE CGRGFSQKSD LIKHQRTHTE EKPYICRECG RGFTQKSDLI KHQRTHTEEK PYICRECGRG FTQKSDLIKH QRTHTGEKPY ICRECGRGFT QKSDLIKHQR THTEEKPYIC RECGRGFTQK SSLIRHQRTH TGEKPYICRE CGLGFTQKSN LIRHLRTHTG EKPYICRECG LGFTRKSNLI QHQRTHTGEK PYICRECGQG LTWKSSLIQH QRTHTGEKPY ICRECGRGFT WKSSLIQHQR THTVEK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Also can methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression. During meiotic progression hotspot-bound PRDM9 interacts with several complexes; in early leptonema binds CDYL and EHMT2 followed by EWSR1 and CXXC1 by the end of leptonema. EWSR1 joins PRDM9 with the chromosomal axis through REC8. In this way, controls the DSB repair pathway, pairing of homologous chromosomes and sex body formation. Moreover plays a central role in the transcriptional activation of genes during early meiotic prophase thanks to H3K4me3 and H3K36me3 enrichment that represents a specific tag for epigenetic transcriptional activation. In addition performs automethylation. Acetylation and phosphorylation of histone H3 attenuate or prevent histone H3 methylation.
  • 基因功能参考文献：
    1. SET7/9 Recruitment to p21 Gene Promoter Was Increased under Diabetic Conditions PMID: 27652271
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily
  • 数据库链接：

    KEGG: rno:365155

    STRING: 10116.ENSRNOP00000060531

    UniGene: Rn.124847